Consulta: creatorFacets:"Margis,Rogerio"
Registros recuperados: 9
Data/hora: 19/05/2024 06:06:01

Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
Provedor de dados: Genetics and Molecular Biology
Autores: Molina,Lorrayne Gomes; Cordenonsi da Fonseca,Guilherme; Morais,Guilherme Loss de; de Oliveira,Luiz Felipe Valter; Carvalho,Joseane Biso de; Kulcheski,Franceli Rodrigues; Margis,Rogerio.
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next generation sequencing; Small RNA; SiRNA; Molecular markers.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200010

MicroRNAs play critical roles during plant development and in response to abiotic stresses
Provedor de dados: Genetics and Molecular Biology
Autores: Lima,Júlio César de; Loss-Morais,Guilherme; Margis,Rogerio.
MicroRNAs (miRNAs) have been identified as key molecules in regulatory networks. The fine-tuning role of miRNAs in addition to the regulatory role of transcription factors has shown that molecular events during development are tightly regulated. In addition, several miRNAs play crucial roles in the response to abiotic stress induced by drought, salinity, low temperatures, and metals such as aluminium. Interestingly, several miRNAs have overlapping roles with regard to development, stress responses, and nutrient homeostasis. Moreover, in response to the same abiotic stresses, different expression patterns for some conserved miRNA families among different plant species revealed different metabolic adjustments. The use of deep sequencing technologies for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNAs; Development; Abiotic stress; Nutrients; Deep sequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600023

Diversity and evolution of plant diacylglycerol acyltransferase (DGATs) unveiled by phylogenetic, gene structure and expression analyses
Provedor de dados: Genetics and Molecular Biology
Autores: Turchetto-Zolet,Andreia Carina; Christoff,Ana Paula; Kulcheski,Franceli Rodrigues; Loss-Morais,Guilherme; Margis,Rogerio; Margis-Pinheiro,Marcia.
Abstract Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes. DGAT1 and DGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of these genes and its relationships with the two main DGAT genes (DGAT1 and DGAT2). According to our analyses DGAT1, DGAT2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triacylglycerol biosynthesis; DGAT; Phylogeny; Gene structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400524

Complete sequence and comparative analysis of the chloroplast genome of Plinia trunciflora
Provedor de dados: Genetics and Molecular Biology
Autores: Eguiluz,Maria; Yuyama,Priscila Mary; Guzman,Frank; Rodrigues,Nureyev Ferreira; Margis,Rogerio.
Abstract Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes. The cp genome of P. trunciflora is 159,512 bp in size, comprising inverted repeats of 26,414 bp and single-copy regions of 88,097 bp (LSC) and 18,587 bp (SSC). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jaboticaba; Myrtaceae; Chloroplast genome; Next-generation sequencing.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500871

Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs
Provedor de dados: Genetics and Molecular Biology
Autores: Waschburger,Edgar; Kulcheski,Franceli Rodrigues; Veto,Nicole Moreira; Margis,Rogerio; Margis-Pinheiro,Marcia; Turchetto-Zolet,Andreia Carina.
Abstract sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membrane lipid. Identifying genes encoding GPAT in a variety of plant species is crucial to understand their involvement in different metabolic pathways and physiological functions. Here, we performed genome-wide and evolutionary analyses of GPATs in plants. GPAT genes were identified in all algae and plants studied. The phylogenetic analysis showed that these genes group into three main clades. While clades...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant lipids; GPAT enzymes; Phylogeny; Evolution; Gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200355

Araucaria angustifolia chloroplast genome sequence and its relation to other Araucariaceae
Provedor de dados: Genetics and Molecular Biology
Autores: Brandão,José Henrique S. G.; Rodrigues,Nureyev F.; Eguiluz,Maria; Guzman,Frank; Margis,Rogerio.
Abstract Araucaria angustifolia is endemic to southern Brazil. Known as Brazilian pine, A. angustifolia is the only native conifer species with economic and social relevance in this country. Due to massive exploitation, it has suffered a significant population decline and currently is classified as critically endangered. This encouraged the scientific community to investigate genetic features in Brazilian pine to increase resources for management and preservation. In this work, RNA-Seq data was used to determine the complete nucleotide sequence of the A. angustifolia chloroplast genome (cpDNA). The cpDNA is 146,203 bp in length and contains 122 genes, including 80 protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes. Coding regions comprise...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian pine; Plastid; Genome; CpDNA; Conservation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400671

Transcriptomics analysis of Psidium cattleyanum Sabine (Myrtaceae) unveil potential genes involved in fruit pigmentation
Provedor de dados: Genetics and Molecular Biology
Autores: Vetö,Nicole M.; Guzman,Frank; Kulcheski,Franceli R.; Segatto,Ana Lúcia A.; Lacerda,Maria Eduarda G.; Margis,Rogerio; Turchetto-Zolet,Andreia C.
Abstract Psidium cattleyanum Sabine is an Atlantic Forest native species that presents some populations with red fruits and others with yellow fruits. This variation in fruit pigmentation in this species is an intriguing character that could be related to species evolution but still needs to be further explored. Our goal was to provide genomic information for these morphotypes to understand the molecular mechanisms of differences in fruit colour in this species. In this study, we performed a comparative transcriptome analysis of red and yellow morphotypes of P. cattleyanum, considering two stages of fruit ripening. The transcriptomic analysis performed encompassing leaves, unripe and ripe fruits, in triplicate for each morphotype. The transcriptome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthocyanin; Atlantic Forest; Carotenoids; Fruit ripening; Myrtaceae.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400803

Comparative analysis of the complete chloroplast genomes from six Neotropical species of Myrteae (Myrtaceae)
Provedor de dados: Genetics and Molecular Biology
Autores: Rodrigues,Nureyev F.; Balbinott,Natalia; Paim,Igor; Guzman,Frank; Margis,Rogerio.
Abstract Myrteae is the largest and most diverse tribe within Myrtaceae and represents the majority of its diversity in the Neotropics. Members of Myrteae hold ecological importance in tropical biomes for the provision of food sources for many animal species. Thus, due to its several roles, a growing interest has been addressed to this group. In this study, we report the sequencing and de novo assembly of the complete chloroplast (cp) genomes of six Myrteae species: Eugenia brasiliensis, E. pyriformis, E. nitida, Myrcianthes pungens, Plinia edulis and Psidium cattleianum. We characterized genome structure, gene content, and identified SSRs to detect variation within Neotropical Myrteae. The six newly sequenced plastomes exhibit a typical quadripartite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CpDNA; Genomic resource; Populational genetics; Plastid; Conservation.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400801

Gene stacking as a strategy to confer characteristics of agronomic importance in plants by genetic engineering
Provedor de dados: Ciência Rural
Autores: Ceccon,Cássia Canzi; Caverzan,Andréia; Margis,Rogerio; Salvadori,José Roberto; Grando,Magali Ferrari.
ABSTRACT: Gene stacking refers to the introduction of two or more transgenes of agronomic interest in the same plant. The main methods for genetically engineering plants with gene stacking involve (i) the simultaneous introduction, by the co-transformation process, and (ii) the sequential introduction of genes using the re-transformation processes or the sexual crossing between separate transgenic events. In general, the choice of the best method varies according to the species of interest and the availability of genetic constructions and preexisting transgenic events. We also present here the use of minichromosome technology as a potential future gene stacking technology. The purpose of this review was to discuss aspects related to the methodology for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-transformation; Re-transformation; Sexual crossing; Transgene; Minichromosomes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000600401